492029
NRG1-β1/HRG1-β1 EGF Domain, Human, Recombinant
Assay
≥97% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, human neuregulin 1-β1/heregulin 1-β1 epidermal growth factor-like domain (NRG1-β1/HRG1-β1 EGF Domain), consisting of amino acids 176-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain that is required for binding directly to the ErbB3 or ErbB4 receptor tyrosine kinases.
Biochem/physiol Actions
ED₅₀ = 0.06-0.3 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.
Physical form
Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Other Notes
Buonanno, A., and Fischbach, G.D. 2001. Curr. Opin. Neurobiol.11, 287.
Adlkofer, K. and Lai, C. 2000. Glia29, 104.
Garratt, A.N. et al. 2000. BioEssays22, 987.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Adlkofer, K. and Lai, C. 2000. Glia29, 104.
Garratt, A.N. et al. 2000. BioEssays22, 987.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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