Oncostatin M, Human, Recombinant, E. coli

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposi′s sarcoma cells, and human erythroleukemia cell line, TF-1.

ED₅₀ = 0.15-0.30 ng/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg rhOSM.

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% BSA or HSA.

Boileau, C., et al. 2000. J. Immunol.164, 5713.
Miyajima, A., et al. 2000. Cytokine Growth Factor Rev.11, 177.
Linsley, P.S., et al. 1990. Mol. Cell. Biol.10, 1882.
Kitamura, T., et al. 1989. J. Cell Physiol.140, 323.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)