507858
PANSORBIN® Cells
Synonym(s):
Staphylococcus aureus Cells
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About This Item
NACRES:
NA.42
UNSPSC Code:
41116133
Manufacturer/tradename:
Calbiochem®
form
suspension (Homogeneous)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
impurities
≤2500 cells/mL Viable cells
color
milky
capacity
≥2.0 mg(human IgG/100 mg cells)
shipped in
ambient
Quality Level
General description
PANSORBIN Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.
Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
Physical form
Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
Other Notes
Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
高风险级别生物产品-Merck
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