A cell-permeable, photostable, bodipy-derived fluorescent probe that can be used for real-time imaging of lysosomal dynamics in proliferating cells undergoing mitosis. Undergoes rapid re-localization from cytoplasm to nucleus during cell division, but does not interfere with cell cycle. Displays a sharp increase in intensity during mitosis and its fluorescence displays high sensitivity to acidic environment (pH 2-4). Can also be used for time-dependent whole-body in vivo imaging of tumor xenografts (CDy6 stained HeLa cells, s.c.) in BALB/c nude mice up to 21 days. Exhibits greater brightness, higher photostability, and significantly reduced toxicity when compared to LysoTracker Red, LysoTracker Green, LysoSensor Green and HOE33342. Abs _max: 570 nm; Em _max: 585 with a quantum yield of 0.89.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Cell permeable: yes

Primary Target
cytoplasm

Reversible: yes

Packaged under inert gas

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C). Store in a dark vial, protected from light.

Use only fresh, anhydrous DMSO for reconstitution.

Jeong, Y.M., et al. 2015. Chem. Biol.22, 299.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany