539131
Protease Inhibitor Cocktail I
lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases
Synonym(s):
Protease Inhibitor Mix, Protease inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.28
Form:
lyophilized
Solubility:
water: soluble
Storage temp.:
−20°C
Product Name
Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
General description
Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.
Application
Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
- cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
- RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
- sucrose solution to homogenize mice cortices for myelin isolation
Biochem/physiol Actions
Inhibits a broad range of proteases and esterases.
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.
Features and Benefits
- Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
- Ready for reconstitution
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.
Other Notes
The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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