Quality Level
Assay
≥95% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS.
Biochem/physiol Actions
Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Stein, R.L., et al. 1996. Biochemistry 35, 3899.
Ciechanover, A. 1994. Cell 79, 13.
Dick, L.R., et al. 1994. J. Immunol. 152, 3884.
Dick, L.R., et al. 1991. Biochemistry 30, 2725.
Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.
Ciechanover, A. 1994. Cell 79, 13.
Dick, L.R., et al. 1994. J. Immunol. 152, 3884.
Dick, L.R., et al. 1991. Biochemistry 30, 2725.
Hough, R., et al. 1987. J. Biol. Chem. 262, 8303.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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