Skip to Content
Merck
CN

539482

Sigma-Aldrich

PKA, His•Tag®, Active, Human, Recombinant, E. coli

Synonym(s):

Protein Kinase A, cAMP Dependent Protein Kinase

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352202

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Assay

≥95% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥500,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recombinant, human, catalytic subunit of PKA fused to at the N-terminus to a His•Tag sequence and expressed in E.coli. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for labeling PKA substrates.

Recombinant, human, catalytic subunit of PKA fused to at the N-terminus to a His•Tag sequence and expressed in E. coli. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for labeling PKA substrates.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM KCl, 30 mM K₂HPO₄, 1 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to PKA peptide (GRTGRRNSI) per min at 30°C.
Skalhegg, B.S. and Tasken K., et al. 2000. Front. Biosci.5, D678.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

10-13 - German Storage Class 10 to 13

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service