Assay
≥95% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥8,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
color
clear colorless
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human PP2Cα fused to a His•Tag sequence and expressed in E. coli. A Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response, gene expression and replication in eukaryotes. Recently it has been shown that PP2Cα inhibits cell growth and activates the p53 pathway.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Ofek, P., et al. 2003. J. Biol. Chem.278, 14299.
Das, A.K., et al. 1996. EMBO J.15, 6798.
Das, A.K., et al. 1996. EMBO J.15, 6798.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 37°C, pH 7.4 using 10 mM of substrate.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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