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Sigma-Aldrich

Normal Donkey Serum, Sterile

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form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

−20°C

Related Categories

General description

Normal carrier sera, produced from pooled lots of sera from healthy, non-immunized donkeys.
Normal donkey serum is a common blocking agent used to reduce background staining by preventing non-specific binding of antibodies to tissue or fragment crystallizable (FC) receptors.

Application

Normal Donkey Serum, Sterile has been used to reduce artificial staining of non-specific proteins during double-immunofluorescent labeling. It is also suitable for use in radioimmunoassay.

Warning

Toxicity: Standard Handling (A)

Physical form

pH 7.0-8.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Woo Jung Cho et al.
Journal of cellular and molecular medicine, 11(5), 1069-1086 (2007-11-06)
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon
Igor Buchwalow et al.
Scientific reports, 1, 28-28 (2012-02-23)
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background staining is thought to occur as a result of either non-specific antibody (Ab) binding to endogenous Fc receptors (FcRs) or a combination of ionic and
R F Holcombe et al.
Molecular pathology : MP, 55(4), 220-226 (2002-07-31)
Signalling through the Wnt pathway is integrally associated with colon carcinogenesis. Although activating mutations in the genes for adenomatous polyposis coli (APC) and beta-catenin are clearly associated with colon cancer, less is understood about the role of the upstream secreted

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