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688002-M

Y-27632

Name: Y-27632
Brand: MM

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem^®

Synonym(s):

InSolution Y-27632 in DMSO, Rho Kinase Inhibitor VI, (R)-(+)- trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₂₁N₃O · 2HCl · xH₂O

Molecular Weight:

320.26 (anhydrous basis)

UNSPSC Code:

12352200

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

liquid

Quality level:

100

Storage condition:

OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light

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Properties

Product Name

Y-27632 in DMSO, InSolution, ≥95%, 10 mM, reversible and selective inhibitor of Rho-associated protein kinases

Quality Level

100

assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light

storage temp.

−70°C

Description

General description

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160^ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency.

Physical form

A 10 mM (1 mg/296 µl) solution of Y-27632 (Cat. No. 688000)in DMSO.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Watanabe, K., et al. 2007. Nature Biotech.25, 681.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1998. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science285, 895.
Uehata, M., et al. 1997. Nature389, 990.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - (Dimethylsulfoxide)

flash_point_c

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

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Global Trade Item Number

SKU	GTIN
688002-1MG	04055977225464