antibody form
purified antibody
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Sensitive, specific detection of Nus•Tag fusion proteins
The Nus•Tag<TMSYMBOL></TMSYMBOL> Monoclonal Antibody is a mouse monoclonal antibody (IgG1) with high affinity for the 54.8-kDa NusA protein from E. coli. This purified antibody specifically detects fusion proteins containing the Nus•Tag sequence expressed with the pET-43.1 and pET-44 vector series. Expression of polypeptides fused with the 495-aa NusA (Nus•Tag) can markedly enhance the solubility of recombinant proteins expressed in E. coli (Harrison 2000, Davis 1998).
The 50-g package size provides enough purified antibody to perform 50 Western blots (10 cm x 10 cm).
The 50-g package size provides enough purified antibody to perform 50 Western blots (10 cm x 10 cm).
Other Notes
Harrison, R.G. 2000. inNovations11, 4.
Davis, G.D., et al. 1998. Biotechnol. Bioeng.65, 382.
Davis, G.D., et al. 1998. Biotechnol. Bioeng.65, 382.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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