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Merck
CN

IPVH85R

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Synonym(s):

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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Product Name

Immobilon®-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

Quality Level

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon®-P transfer membrane is a hydrophobic polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is used for the transfer of proteins from a variety of gel matrices. Immobilon®-P transfer membrane is recommended for most western blotting, especially proteins greater than 20 kDa. This membrane displays improved handling characteristics and staining capabilities, a higher signal-to-noise ratio for enhanced sensitivities, and improved solvent resistance, when compared to nitrocellulose membrane.
Primary Binding Mechanisms: Electrostatic, hydrophobic,
Filter Code: IPVH

Application

Immobilon®-P Membrane, PVDF, 0.45 μm, 8.5 cm x 10 m roll has been used in western blot analysis and co-immunoprecipitation.

Other Notes

Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany




Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

View All Protocols

Articles

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

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Global Trade Item Number

SKUGTIN
IPVH85R04054839569067