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MCKP1-110K-PX28

MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1

Premixed Mouse Immuno-Oncology Checkpoint Protein 28-Plex Panel 1

Synonym(s):

Luminex mouse immuno oncology panel, checkpoint inhibitor multiplex assay, mouse IO panel, mouse cancer immunotherapy assay, mouse immune checkpoint profiling kit

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503
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Product Name

MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1,

description

Premixed Mouse Immuno-Oncology Checkpoint Protein 28-Plex Panel 1

Quality Level

200

species reactivity

mouse

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

inter-assay cv: <20%
intra-assay cv: <10%, accuracy: 101
(Galectin-1), accuracy: 103
(CD80/B7-1), accuracy: 103
(IFNγ), accuracy: 104
(CTLA-4/CD152), accuracy: 104
(PD-L2), accuracy: 104
(TLR-2), accuracy: 105
(4-1BBL/TNFSF9), accuracy: 105
(BCA-1/CXCL-13), accuracy: 105
(CD25/IL-2Rα), accuracy: 105
(IL-10), accuracy: 105
(LAG-3), accuracy: 106
(5’-NT/CD73), accuracy: 106
(PD-1), accuracy: 106
(TIM-3), accuracy: 106
(TNFα), accuracy: 107
(CD137/4-1BB), accuracy: 107
(CD27), accuracy: 108
(E-Cadherin), accuracy: 108
(Galectin-3), accuracy: 108
(HVEM), accuracy: 109
(CD40), accuracy: 109
(GITR), accuracy: 110
(B7-H2/ICOSL), accuracy: 110
(CD226/DNAM-1), accuracy: 111
(BTLA), accuracy: 94
(GranzymeB), accuracy: 95
(B7-H3/CD276), accuracy: 96
(PD-L1), standard curve range: 1.4 - 1000 pg/mL
(HVEM), standard curve range: 11 - 8000 pg/mL
(IL-10), standard curve range: 137 - 100000 pg/mL
(5’-NT/CD73), standard curve range: 14 - 10000 pg/mL
(4-1BBL/TNFSF9), standard curve range: 14 - 10000 pg/mL
(CD226/DNAM-1), standard curve range: 14 - 10000 pg/mL
(CD40), standard curve range: 14 - 10000 pg/mL
(PD-L1), standard curve range: 2.1 - 1500 pg/mL
(IFNγ), standard curve range: 2.7 - 2000 pg/mL
(CD25/IL-2Rα), standard curve range: 21 - 15000 pg/mL
(BTLA), standard curve range: 27 - 20000 pg/mL
(B7-H2/ICOSL), standard curve range: 27 - 20000 pg/mL
(BCA-1/CXCL-13), standard curve range: 3.4 - 2500 ng/mL
(B7-H3/CD276), standard curve range: 3.4 - 2500 ng/mL
(Galectin-1), standard curve range: 34.3 - 25000 pg/mL
(CD80/B7-1), standard curve range: 342 - 250000 pg/mL
(E-Cadherin), standard curve range: 4.1 - 3000 pg/mL
(TNFα), standard curve range: 41 - 30000 pg/mL
(GITR), standard curve range: 41 - 30000 pg/mL
(Galectin-3), standard curve range: 412 - 300000 pg/mL
(LAG-3), standard curve range: 412 - 300000 pg/mL
(TLR-2), standard curve range: 5.5 - 4000 pg/mL
(CD137/4-1BB), standard curve range: 5.5 - 4000 pg/mL
(CTLA-4/CD152), standard curve range: 5.5 - 4000 pg/mL
(TIM-3), standard curve range: 55 - 40000 pg/mL
(CD27), standard curve range: 55 - 40000 pg/mL
(PD-1), standard curve range: 6.9 - 5000 pg/mL
(PD-L2), standard curve range: 8.2 - 6000 pg/mL
(GranzymeB)

technique(s)

multiplexing: suitable

input

serum
plasma
cell culture supernatant(s)

detection method

fluorometric (Luminex® xMAP®technology)

shipped in

wet ice

storage temp.

2-8°C

General description

Immune checkpoint proteins are key regulators of the immune system and drug targets of cancer immunotherapy. Immune checkpoint molecules have been found on the cell surface of immune cells and tumor cells as activating receptors or inhibitory receptors. Immune checkpoint inhibitors, such as blocking antibodies to cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), programmed cell death protein-1 (PD-1) and programmed cell death ligand 1 (PD-L1), have demonstrated significant efficacy in the treatment of a variety of cancers. Recent studies have also shown many immune checkpoint proteins can undergo alternative splicing or proteolytic cleavage to produce soluble checkpoint proteins. The expression levels of soluble and cell-surface immune checkpoint proteins in the circulation and in tumor-microenvironment (TME) may be used as putative immune-based biomarkers for cancer research and biomarker discovery in immunological diseases.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 28 analytes in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.

Analytes included: 4-1BBL/TNFSF9, 5′-NT/CD73, B7-H2/ICOSL, B7-H3/CD276, BCA-1/CXCL-13, BTLA, CD137/4-1BB, CD226/DNAM-1, CD25/IL-2Rα, CD27, CD40, CD80/B7-1, CTLA-4/CD152, E-cadherin, Galectin-1, Galectin-3, GITR, Granzyme B, HVEM, IFNγ, IL-10, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2, and TNFα

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

Enhanced Research Efficiency: By measuring multiple proteins at once, researchers can gain insights into immune responses without running multiple individual assays.

Improved Data Quality: High sensitivity and specificity lead to more reliable data, which is critical for translational research.

Facilitates Drug Development: Understanding checkpoint protein interactions can aid in the development of more effective immunotherapies.

Supports Biomarker Discovery: Helps identify potential biomarkers for sample stratification in immunotherapy research.

Comprehensive Analysis: Provides a broader view of the immune environment in tumors, aiding in the understanding of tumor-immune interactions.

Legal Information

Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Regulatory Information

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