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PCHT06H48

Millicell® 6 well hanging cell culture inserts

pore size 0.4 μm, clear PET membrane, diam. 24 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

permeable inserts, tissue culture inserts, transparent membrane insert, imaging insert

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About This Item

NACRES:
NB.13
UNSPSC Code:
41122107
Material:
clear PET membrane, polystyrene housing
Pore size:
0.4 μm
Sterility:
γ-irradiated, sterile
Binding type:
Tissue Culture (TC)-treated surface
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Quality Level

200

material

clear PET membrane, polystyrene housing

sterility

γ-irradiated, sterile

feature

4x106 pores/cm2 (pore density)

packaging

pack of 48 ea (individually blister packaged)

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable, cell differentiation: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, permeability assay: suitable

H

15.5 mm

diam.

24 mm

insert maximum volume

4 mL

insert working volume

2 mL

membrane thickness

9-16 μm

size

6 wells

surface area

4.5 cm2

color

clear membrane, transparent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an optically clear 0.4 μm membrane pore size and fits in most 6-well tissue culture plates.

Application

Barrier Assays
Cell Proliferation
Cell Surface Receptors
Co-Culture
Epithelial Cell Growth
Feeder Layers
Fluorescent Detection /Immunohistochemistry
In Vitro Fertilization
In Vitro Toxicology
Microbial Attachment
Phase Contrast Microscopy
Polarized Protein Secretions
Polarized Uptake Transport/Permeability

Features and Benefits

Optically clear TC-treated PET membrane
Low pore density (4 million pores/cm2)
Compatible with most 6-well plates
Apical assist rim to prevent membrane puncture or disruption of cells
Easily access the basolateral side between the flanges

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Barrier Formation and Permeability Assays using Millicell® Hanging Cell Culture Inserts

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

Validated Cell Types for Millicell® DCI Digital Cell Imager

Cell validation data using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.