AAMT001
Mitochondrial Complex I Activity Assay Kit
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About This Item
UNSPSC Code:
41181505
manufacturer/tradename
Novagen®
storage condition
OK to freeze
storage temp.
2-8°C
General description
This product has been discontinued.
Product Description
The Mitochondrial Complex I Activity Assay Kit (Cat. No. AAMT001-1KIT) is used to determine the activity of mitochondrial OXPHOS Complex I (NADH dehydrogenase, E.C. 1.6.5.3). The enzyme complex is immunocaptured within the wells of the microplate and activity is determined by following the oxidation of NADH to NAD+ and the simultaneous reduction of a dye which leads to increased absorbance at 450 nm.
See Figure 1 at the bottom of this page for the Complex I activity reaction scheme. The oxidation of NADH is linked to the reduction of a dye which has increased absorbance at 450 nm and so appears yellow in color.
Panel Components
Storage: Store all components at 4°C. After hydration of the 20X NADH and 100X dye with dH20, both of these components should be stored be aliquotted and stored at –80°C, as described in the FAQ section of TB554.
This kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitochondrial Complex I Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
Product Description
The Mitochondrial Complex I Activity Assay Kit (Cat. No. AAMT001-1KIT) is used to determine the activity of mitochondrial OXPHOS Complex I (NADH dehydrogenase, E.C. 1.6.5.3). The enzyme complex is immunocaptured within the wells of the microplate and activity is determined by following the oxidation of NADH to NAD+ and the simultaneous reduction of a dye which leads to increased absorbance at 450 nm.
See Figure 1 at the bottom of this page for the Complex I activity reaction scheme. The oxidation of NADH is linked to the reduction of a dye which has increased absorbance at 450 nm and so appears yellow in color.
Panel Components
25 ml Buffer, (72560-25ML)10 ml 10X Blocking Solution, (72561-10ML)1 ml Detergent, (72562-1ML)1 ea 20X NADH (40 mM), (72563-1EA)1 ea 100X Dye, (72564-1EA)1 ea Microplate, (72565-1EA)* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store all components at 4°C. After hydration of the 20X NADH and 100X dye with dH20, both of these components should be stored be aliquotted and stored at –80°C, as described in the FAQ section of TB554.
This kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitochondrial Complex I Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Regulatory Information
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