Anti-Tie-1 Antibody, CT

20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1.

Epitope: C-terminus

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-Tie-1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Tie-1.

Western blot: 1-10 μg/mL using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140 kDa.

ELISA: (1:10,000 -1:100,000) using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Recognizes human Tunica internal endothelial cell kinase (Tie-1), also described as tyrosine protein kinase receptor. Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.

SPECIES REACTIVITIES: The peptide sequence used is 100% conserved in mouse, rat, and bovine. Antibody crossreactivity in other species is not established.

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide.

Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze and thaw.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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