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AB3184

Sigma-Aldrich

Anti-MEK5 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

Xenopus, canine, rabbit, sheep, monkey, human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_002757.2
NM_145160.1

UniProt accession no.

Q13163

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MAP2K5(5607)
mouse ... Map2K5(23938)

Specificity

MEK 5 is also known as MAP Kinase Kinase 5, or ERK Kinase 5. AB3184 detects a 49 kDa protein, corresponding to the expeted molecular mass of Mek5, in rat brain, rat liver, rat kidney, rat heart, mouse brain, and mouse testis tissue extracts as well as in cell lysates prepared from the rat pheochromacytoma (PC12) and the human HeLa cell lines. No cross-reactivity with MEK 1, 2, 3, 4 or 6 has been observed with this antibody.

Immunogen

Human MEK 5 synthetic peptide (aa. 59-74; Genbank Accession # U71088), conjugated to KLH; identical to rat MEK5 sequence over these residues.

Application

Anti-MEK5 Antibody is an antibody against MEK5 for use in ELISA, IH, IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases
Western Blot: 1 μg/mL

Immunohistochemistry: 1:1,000

Immunoprecipitation

EIA

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Affinity purified immunoglobulin

Liquid containing PBS, pH 7.2. No preservatives.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Thawed product may be stored at 2-8°C for up to 4 weeks. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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