Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196

Corresponds to the C-terminus of the longer form of BKCa channel a subunit and overlaps by >40 residues with a shorter form.

A purified fusion protein (GST)1 and a C-terminal part (residues 1097-1196) of mouse Slo (mSlo) alpha subunit of BKCa channel.

Epitope: C-terminal sequence SHSSHSSQ

Anti-Potassium Channel MaxiK Antibody, calcium activated, aa1097-1196 detects level of Potassium Channel MaxiK & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL

Dilutions should be made using a carrier protein such as BSA (1-3%). Typical bands detected are ~125kDa in rat membrane preparations. Excellent methods given in Ling, et al (2003) {http://jp.physoc.org/cgi/content/full/553/1/65}

Optimal working dilutions must be determined by the end user.

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blotting, use 20 ng protein per lane. For negative control, preincubate 3-5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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