The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Epitope: blue

Recombinant human blue opsin.

Immunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

This Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.

Recognizes Opsin, blue.

Format: Purified

Protein A purified

Purified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative

Maintain for 1 year at 2–8°C from date of shipment.

Control
Retina

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