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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable

suitability

not suitable for Western blot

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

shipped in

dry ice

Gene Information

human ... COL1A1(1277)

Immunogen

purified bovine type I collagen extracted from purified bovine skin.

Application

Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.

Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.

Radioimmunoassay

ELISA: 1:200 direct

Not recommended for Western blots.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

Reacts with bovine collagen type I collagen.

Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.

Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.

Physical form

Format: Purified
Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.

Preparation Note

Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB749

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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