The mammalian Trk family of receptor tyrosine kinases consists of three principle transmembrane proteins. The receptors can be activated by one or more of four neurotrophins - NGF, BDNF, NT3 and NT4/5. The TrkB family of transmembrane proteins serves as receptors for BDNF and NT-4/5 and these neurotrophins regulate biological responses such as cell survivial, proliferation and differentiation in normal and neoplastic neuronal cells.

Extracellular domain of the recombinant mouse receptor expressed in mammalian cells.

Anti-TrkB Antibody is an antibody against TrkB for use in IH.

Immunohistochemistry: 1:3,000. The antibody has been used successfully on rat dorsal root ganglion and trigeminal ganglia in the rat spinal cord (cryostat free-floating sections). Suggested fixative is 4% paraformaldehyde.

Not suitable for Western blot.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Other species have not yet been tested. It is expected to react with a wide range of mammalian species, based on the high sequence similarity.

trkB [BDNF/NT-4/5 growth factor receptor; tyrosine kinase receptor B]. The immunogen sequence was chosen to avoid cross reactivity with trkA or trkC.

Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.

Serum

Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.

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