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Merck
CN

AB9900

Anti-IDO Antibody

Chemicon®, from rabbit

Synonym(s):

Indoleamine 2,3-dioxygenase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, WB
Citations:
3

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, western blot: suitable

NCBI accession no.

NM_002164.4

UniProt accession no.

P14902

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Ido1(15930)

General description

45 kDa

Immunogen

Recombinant mouse IDO.

Application

Anti-IDO Antibody detects level of IDO & has been published & validated for use in ELISA & WB.
Western blot: 1:2,000-1:5,000 using ECL. Reacts with the ~45 kDa IDO protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:2,000-1:10,000.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

IDO [Indoleamine 2,3-dioxygenase], mouse.

Physical form

Format: Purified
Liquid on PBS, pH 7.4.

Other Notes

Replaces: 04-1056

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christoph-Martin Ufermann et al.
Frontiers in cellular and infection microbiology, 9, 184-184 (2019-06-25)
Toxoplasma gondii (T. gondii) is an obligate intracellular parasite and belongs to the phylum Apicomplexa. T. gondii is of medical and veterinary importance, because T. gondii causes the parasitic disease toxoplasmosis. In human cells, the interferon-gamma inducible indoleamine 2,3-dioxygenase 1
Activation of the alternative NF?B pathway improves disease symptoms in a model of Sjogren's syndrome.
Gilboa-Geffen, A; Wolf, Y; Hanin, G; Melamed-Book, N; Pick, M; Bennett, ER; Greenberg et al.
Testing null
Giorgia Renga et al.
Nature communications, 14(1), 5753-5753 (2023-09-17)
The aromatic amino acid L-tryptophan (Trp) is essentially metabolized along the host and microbial pathways. While much is known about the role played by downstream metabolites of each pathways in intestinal homeostasis, their role in lung immune homeostasis is underappreciated.

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