Anti-phospho-Rb (Ser807/Ser811) Antibody

The retinoblastoma (Rb) gene product (also known as p105, p110 or pRb) is a tumor suppressor protein that is missing or mutated in a variety of human tumors. In the hereditary childhood form of retinoblastoma, Rb is lost from chromosome 13. Rb regulates cell proliferation and differentiation by controlling progression through the restriction point within the G1 phase of the cell cycle. Rb forms complexes with many proteins, and this binding activity is required for growth suppression. Rb specifically interacts with the E2F family of transcription factors to repress E2F-mediated transcription. Rb also interacts and alters the activity of other transcription factors including Elf-1, ATF-2, MyoD, BRG-1, c-myc, N-myc. Rb recruits a histone deacetylase, HDAC1, to E2F-1 to repress transcription. Two Rb related proteins p107 and p130 also function to regulate specific members of the E2F transcription factor family.

~110 kDa observed

Epitope: Phosphorylated Ser807/Ser811

KLH-conjugated linear peptide corresponding to human Rb phosphorylated at Ser807/Ser811.

Anti-phospho-Rb (Ser807/Ser811) Antibody is an antibody against phospho-Rb (Ser807/Ser811) for use in WB, Cell Function Assay.

Peptide Inhibition Assay Analysis: 0.05 µg/mL from a representative lot peptide blocked on MOLT4 cells stimulated with PMA.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

11/12 amino acids identical in Rat and chicken Rb

This antibody recognizes Rb phosphorylated at Ser807/Ser811.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA

Evaluated by Western Blot in untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Rb on 10 µg of untreated and lambda phosphatase-treated MOLT4 cell lysates stimulated with PMA.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.