ABE171
Anti-MeCP2 Antibody
from chicken, purified by affinity chromatography
Synonym(s):
methyl CpG binding protein 2 (Rett syndrome), mental retardation, X-linked 16, MeCp-2 protein, mental retardation, X-linked 79, methyl-CpG-binding protein 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
chicken
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgY
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MECP2(4204)
General description
MeCp2 (methyl-CpG-binding protein 2) binds methylated CpG sequences in double-stranded DNA resulting in the suppression of transcription. Although MeCp2 is not essential in stem cells, it is critical for embryonic development. This protein seems to be particularly important for mature nerve cells, where it is present in high levels. The MeCp2 protein is likely to be involved in turning off ("repressing" or "silencing") several other genes. Consistent with this role, mutations of MeCp2 are linked to Rett syndrome (RTT), a progressive neurological disorder that is one of the most common causes of mental retardation in females.
~75 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human MeCp2.
Application
Anti-MeCP2 Antibody is a Chicken Polyclonal Antibody for detection of MeCP2 also known as methyl CpG binding protein 2 (Rett syndrome), mental retardation X-linked 16 & has been validated in WB.
Chromatin Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory in ChIP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Physical form
Antigen Affinity Purified
Purified chicken polyclonal in buffer with 0.05% sodium azide and 50% glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
SH-SY5Y nuclear extract
SH-SY5Y nuclear extract
Evaluated by Western Blot in SH-SY5Y nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected MeCp2 on 10 µg of SH-SY5Y nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected MeCp2 on 10 µg of SH-SY5Y nuclear extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Leslie Rietveld et al.
Frontiers in cellular neuroscience, 9, 145-145 (2015-05-06)
Rett syndrome (RTT) is a progressive neurological disorder primarily caused by mutations in the X-linked gene methyl-CpG-binding protein 2 (MECP2). The heterozygous female brain consists of mosaic of neurons containing both wild-type MeCP2 (MeCP2+) and mutant MeCP2 (MeCP2-). Three-dimensional morphological
Marissa Sobolewski et al.
Toxicological sciences : an official journal of the Society of Toxicology, 163(2), 478-489 (2018-02-27)
Developmental exposure to lead (Pb) and prenatal stress (PS) both impair cognition, which could derive from their joint targeting of the hypothalamic-pituitary-adrenal axis and the brain mesocorticolimbic (MESO) system, including frontal cortex (FC) and hippocampus (HIPP). Glucocorticoids modulate both FC
Xiaorui Wang et al.
Diabetologia, 57(1), 236-245 (2013-10-01)
Pro-opiomelanocortin (POMC) neurons in the arcuate nucleus (ARC) regulate energy homeostasis by secreting α-melanocyte-stimulating hormone (α-MSH), derived from POMC precursor, in response to leptin signalling. Expression of Pomc is subject to multiple modes of regulation, including epigenetic regulation. Methyl-CpG-binding protein
Liraz Keidar et al.
Frontiers in cellular neuroscience, 13, 370-370 (2019-09-03)
LIS1 is the main causative gene for lissencephaly, while MeCP2 is the main causative gene for Rett syndrome, both of which are neurodevelopmental diseases. Here we report nuclear functions for LIS1 and identify previously unrecognized physical and genetic interactions between
James C Cronk et al.
Immunity, 42(4), 679-691 (2015-04-23)
Mutations in MECP2, encoding the epigenetic regulator methyl-CpG-binding protein 2, are the predominant cause of Rett syndrome, a disease characterized by both neurological symptoms and systemic abnormalities. Microglial dysfunction is thought to contribute to disease pathogenesis, and here we found
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