MeCp2 (methyl-CpG-binding protein 2) binds methylated CpG sequences in double-stranded DNA resulting in the suppression of transcription. Although MeCp2 is not essential in stem cells, it is critical for embryonic development. This protein seems to be particularly important for mature nerve cells, where it is present in high levels. The MeCp2 protein is likely to be involved in turning off ("repressing" or "silencing") several other genes. Consistent with this role, mutations of MeCp2 are linked to Rett syndrome (RTT), a progressive neurological disorder that is one of the most common causes of mental retardation in females.

~75 kDa observed

KLH-conjugated linear peptide corresponding to human MeCp2.

Anti-MeCP2 Antibody is a Chicken Polyclonal Antibody for detection of MeCP2 also known as methyl CpG binding protein 2 (Rett syndrome), mental retardation X-linked 16 & has been validated in WB.

Chromatin Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory in ChIP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Thatcher, K., et al. (2005). Hum. Mol. Genet. 14(6): 785-797.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Antigen Affinity Purified

Purified chicken polyclonal in buffer with 0.05% sodium azide and 50% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
SH-SY5Y nuclear extract

Evaluated by Western Blot in SH-SY5Y nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected MeCp2 on 10 µg of SH-SY5Y nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.