Anti-N-CoR Antibody

~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa

In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.

KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.

Anti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
RAW 264.7 cell lysate

Evaluated by Western Blot in RAW 264.7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 06-892

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