Anti-BMAL1

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: O00327 also known as Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, BMAL1, Class E basic helix-loop-helix protein 5, bHLHe5; Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3) is encoded by the ARNTL (also known as BHLHE5; BMAL1; MOP3; PASD3) gene (gene ID 406) in human. BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart. It is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 is shown to positively regulate myogenesis and negatively regulate adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. BMAL1 is the only component of the mammalian circadian clock whose deletion in a murine model is shown to generate arrhythmicity. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and they age quickly.

Detects BMAL1 (Aryl hydrocarbon receptor nuclear translocator-like protein 1) in HeLa cell nuclear extract.

Epitope: C-terminus

KLH-conjugated peptide corresponding to the C-terminal 20 amino acids of human BMAL1 protein.

Anti-BMAL1, Cat. No. ABE2627, is a highly specific rabbit polyclonal antibody that targets BMAL1 (Aryl hydrocarbon receptor nuclear translocator-like protein 1) and has been validated for use in Immunoprecipitation and Western Blotting

Immunoprecipitation Analysis: A representative lot immunoprecipitated BMAL1 in U2OS cells (Michael, A.K., et al. (2015). Mol Cell. 58(5):743-754).

Western Blotting Analysis: A representative lot detected BMAL1 protein expression in circadian synchronized NCI-H1299 cells (Michael, A.K., et al. (2015). Mol Cell. 58(5):743-754).

Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected BMAL1 in 10 µg of HeLa nuclear extract.

~70 kDa observed. 68.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity purified.

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.