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Merck
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ABE2902

Anti-Cdc45

from rabbit

Synonym(s):

DNA replication protein Cdc45

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Cdc45, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, hamster, human

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_001233719

UniProt accession no.

Q4U3P7

shipped in

ambient

target post-translational modification

unmodified

Quality Level

100

Gene Information

hamster ... Cdc45(100689362)
human ... CDC45(8318)
mouse ... Cdc45(12544)

Analysis Note

Evaluated by Western Blotting in CHO cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc45 in 10 µg of CHO cell lysate.

Application

Anti-Cdc45, Cat. No. ABE2902, is a highly specific rabbit polyclonal antibody that targets DNA replication protein Cdc45 and has been tested for use in Western Blotting.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdc45 in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected Cdc45 in asynchronous, go synchronized and G1 released CHO cells, immunoprecipitated CDC45 from CHO cells, HeLa and NIH3T3 (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533).

Biochem/physiol Actions

This rabbit polyclonal antibody detects Cdc45 in Human and Murine cells.

General description

DNA replication protein Cdc45 (UniProt: Q4U3P7; also known as Cdc45) is encoded by the CDC45 gene (Gene ID: 100689362) in hamster. Cdc45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. It is required for formation of pre-initiation complexes at the G1/S transition and for activation of replication origins. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. Cdc45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon. Expression of Cdc45 is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells do not display Cdc45 activity whereas highly proliferative cells express high levels of Cdc45.
~65 kDa observed; 65.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

His-tagged full length hamster recombinant Cdc45.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
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