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ABN1367

Anti-Pan DIDO Antibody

Name: Anti-Pan DIDO Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Death-inducer obliterator 1, DATF-1, Death-associated transcription factor 1, DIDO1, DIDO2, DIDO3, DIO-1, hDido1, hDido2, hDido3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, WB

Citations:

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Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

nonhuman primates (based on 100% sequence homology), mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable, western blot: suitable

NCBI accession no.

NP_071388

UniProt accession no.

Q9BTC0

target post-translational modification

unmodified

Gene Information

human ... DIDO1(11083)

Description

General description

Death-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation.

~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates.

Immunogen

KLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue.

This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.

Biochem/physiol Actions

This polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0).

Analysis Note

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DIDO isoforms in 10 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c