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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
conjugate
unconjugated
Quality Level
antibody form
serum
clone
polyclonal
purified by
(unpurified)
species reactivity
human, rabbit, rat, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged Recombinant protein corresponding to rat GRASP65.
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT (Joshi, G., et al. (2013). PNAS. 1-10).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in NRK cells (Tang, D., et al. (2012). Biology Open. 1204-1214).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in CHO cells transfected with WT APP and PS1 or APPswe/PS1ΔE9 mutants, cultured primary hippocampal neurons, and WT CHO cells (Joshi, G., et al. (2013). PNAS. 1-10).
Western Blotting Analysis: A representative lot detected GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT, cells stably expressing GFP-tagged GRASP65 WT, and mouse brain (Joshi, G., et al. (2013). PNAS. 1-10).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in NRK cells (Tang, D., et al. (2012). Biology Open. 1204-1214).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in CHO cells transfected with WT APP and PS1 or APPswe/PS1ΔE9 mutants, cultured primary hippocampal neurons, and WT CHO cells (Joshi, G., et al. (2013). PNAS. 1-10).
Western Blotting Analysis: A representative lot detected GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT, cells stably expressing GFP-tagged GRASP65 WT, and mouse brain (Joshi, G., et al. (2013). PNAS. 1-10).
Quality
Evaluated by Western Blotting in human alzheimer′s brain tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GRASP65 in 10 µg of human alzheimer′s brain tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GRASP65 in 10 µg of human alzheimer′s brain tissue lysate.
Physical properties
~65 kDa observed
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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