Anti-PP2A B subunit isoform B56-gamma Antibody

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform (UniProt Q13362; also known as PP2A B subunit isoform B′-gamma, PP2A B subunit isoform B56-gamma, PP2A B subunit isoform PR61-gamma, PP2A B subunit isoform R5-gamma, Renal carcinoma antigen NY-REN-29) is encoded by the PPP2R5C (also known as KIAA0044) gene (Gene ID 5527) in human. Protein phosphatase 2 (PP2 or PP2A) is a ubiquitously expressed heterotrimeric serine/threonine protein phosphatase with broad substrate specificity, such as Raf, MEK, and AKT. PP2A is composed of a structural A and a catalytic C subunit as the dimeric core enzyme, and a regulatory B subunit. There exist two different A subunits (PPP2R1A & PPP2R1B), two different C subunits (PPP2CA & PPP2CB), and 12 different B subunits (PPP2R2B-D, PPP2R3A-C, PPP2R4, PPP2R5A-E). The enzymatic activity of a catalytic subunit is altered upon binding an A subunit even in the absence of a B subunit. The B subunits are believed to play key roles in controlling the localization and specific activity of the A/C holoenzymes.

This antiserum is also known as M880 (Okamoto, K., et al. (1996). Mol. Cell. Biol. 16(11):6593-6602). Immunogen sequence is 100% present in human PPP2R5C spliced isoform 5 and Gamma-3, with the first amino acid missing in isoform 4 and Gamma-2. PPP2R5C spliced isoform Gamma-3 (UniProt Q13362-1) and Gamma-2 (UniProt Q13362-3) were originally referred to as B′α1 and B′α2, respectively (Tehrani, M.A., et al. (1996). J. Biol. Chem. 271(9):5164-5170). The Immunogen sequence is not present in PPP2R5C isoform Gamma-1 or any of the spliced isoforms of the other 11 types of human PP2A B subunits (PPP2R2B, PPP2R2C, PPP2R2D, PPP2R3A, PPP2R3B, PPP2R3C, PPP2R4, PPP2R5A, PPP2R5B, PPP2R5D, PPP2R5E).

Epitope: C-terminal region.

KLH-conjugated linear peptide corresponding to a C-terminal sequence of human PP2A B subunit isoform B56-gamma.

Anti-PP2A B subunit isoform B56-gamma Antibody is an antibody against PP2A B subunit isoform B56-gamma for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blotting Analysis: A representative lot detected the expression of exogenously transfected human PP2A B56γ subunit in HeLa cells, but not in cells co-transfected with B56γ shRNA (Lee et al., 2010; Zimmerman et al, 2012).
Western Blotting Analysis: A representative lot detected exogenously expressed human B′α1 (PPP2R5C spliced isoform Gamma-3) protein in SF9 insect cells and COS-1 cells, as well as the endogenous rat B′α1 following its isolation by immunopreciptation from a rat fibroblast cell line, clone 6 (Okamoto, K., et al. (1996). Mol. Cell. Biol. 16(11):6593-6602).
Immunocytochemistry Analysis: A representative lot detected differential PP2A B56γ3 subunit cytoplamic and nuclear distribution patterns by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa cells at different stages of cell cycle (Lee, T.Y., et al. (2010). J. Biol. Chem. 285:21567-21580).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human B′α1 (PPP2R5C spliced isoform Gamma-3) from SF9 insect cells and COS-1 cells, as well as the endogenous rat B′α1 from a rat fibroblast cell line, clone 6 (Okamoto, K., et al. (1996). Mol. Cell. Biol. 16(11):6593-6602).

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PP2A B subunit isoform B56-gamma in 10 µg of HeLa cell lysate.

~54 kDa observed. 56.66 kDa (isoform Gamma-2), 61.06 kDa (isoform Gamma-3), 62.76 kDa (isoform 4), 64.29 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates.

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.