Anti-NEDD9 Antibody

Enhancer of filamentation 1, also known as CRK-associated substrate-related protein (CAS-L) or Cas scaffolding protein family member 2 or Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9), or Renal carcinoma antigen NY-REN-12 p105, and encoded by the gene NEDD9 or CASL or CASS2, is a docking protein that plays a central role in coordinating tyrosine kinase mediated signaling for cell adhesion. NEDD9 controls the signals between focal adhesions at the cell periphery and the mitotic spindle in response to growth factors and signals. NEDD9 interacts with integrins and various other cellular proteins including BCAR3 and SH2D3C. It is localized to the cytoplasm in most cells but location can vary depending upon the cell type and function. NEDD9 is also found in cellular projections and lamellipodium and is found in the nucleus depending upon the cellular microenvironment. NEDD9 has multiple isoforms and active proteolytic fragments ranging from 55 kDa to 115 kDa. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle. NEDD9 is highly expressed in most cells and tissues and is typically induced upon cell growth and down regulated in adult tissues such as brain. NEDD9 is processed during the cell cycle into multiple proteolytic fragments that appear to have important functions at specific times during the cell cycle process. In cancer NEDD9 over expression can promote tumor growth and NEDD9 acts directly to stabilize Aurora A kinase which is often over expressed in many cancers. Destabilization of Aurora A via the inhibition or destruction of NEDD9 allows for tumor reduction, thus NEDD9 expression is being examined as a potential cancer marker.

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human near the C-terminus of NEDD9.

Detect Nedd-9 using this rabbit polyclonal antibody, Anti-NEDD9 Antibody validated for use in western blotting.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NEDD9 in 10 µg of HUVEC cell lysate.

~110 kDa observed. The calculated molecular weight of this protein is 92 kDa, but can be observed at ~105-115 kDa due to phosphorylation. (Bradbury, P., et al. (2012). BBA. 290-297). A cleavage form of this prortein may be observed at ~55 kDa (Law, S., et al. (1998). Molecular Cellular Biology. 18(6):3540-3551). Uncharacterized band(s) may appear in some lysates.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

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