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Merck
CN

ABT70

Anti-WASH complex subunit 7 (SWIP) Antibody

from rabbit

Synonym(s):

WASH complex subunit 7

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP
Citations:
-
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... WASHC4(23325)

General description

~135 kDa observed.
IgG heavy chain may be observed at approximately 50 kDa
WASH complex subunit 7 (SWIP) is a component of the WASH complex belonging to the WASH S7 family. The WASH complex is localized at early endosomal subdomains involved in the activation and recruitment the ARP 2/3 complex in order to stimulate actin polymerization and tubulin interaction. Through its interaction with FAM21, WASH plays a regulatory role in the retromer-mediated trafficking of vesicles. It is also involved in the tubule fission, components required in endosomal sorting processes as transport intermediates.

Immunogen

GST-tagged recombinant protein corresponding to human WASH complex subunit 7.

Application

Detect WASH complex subunit 7 (SWIP) using this Anti-WASH complex subunit 7 (SWIP) Antibody validated for use in IP.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Physical form

Format: Purified
Protein G Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7
Evaluated by Immunoprecipitation/Western Blot Analysis in HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Immunoprecipitation/Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit 7 in 10 µg of HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
ABT7004053252642647