Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins, which stage immune responses against pathogenic organisms. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc gamma receptors which interact with sialylated Fc fragments on IgG molecules. The coupling of Cy5 to Anti-Human IgG antibody allows for the visualization of proteins by fluorescent microscopy.

~150 kDa observed.

Purified human IgG.

Research Category
Secondary & Control Antibodies

Research Sub Category
All

This Sheep Anti-Human IgG, Cy5 conjugate, Cat. No. AC112S, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.

This antibody is also available in unconjugated and Cy3-conjugated forms (Cat. Nos. AC112 and AC112C).

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC112), this antibody exhibits strongest immunoreactivity towards whole human IgG molecule (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc) with minimal or no cross-reactivity toward IgG from chicken, guinea pig, hamster, mouse, rabbit, and rat species.

Ammonium sulfate.

Purified sheep polyclonal antibody Cy5 conjugate in PBS with 1.5% BSA and 0.09% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in human B-cell hybridoma SA13.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody immunostained 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human B-cell hybridoma SA13, but not HeLa cells.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.