ACK5030GS
Amicon® Pro Affinity Concentration Kit GST with 30kDa Amicon® Ultra-0.5 Device
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About This Item
material
Amicon Ultra styrene-butadiene device
Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding)
polyethylene cap
polyethylene frit
polypropylene device (for exchange)
polypropylene frit
polypropylene tube (AU0.5; for collection)
polypropylene tube (for collection)
polypropylene tube (holder)
regenerated cellulose membrane
sterility
non-sterile
manufacturer/tradename
Amicon® Pro
parameter
10 mL sample volume
technique(s)
buffer exchange: suitable
centrifugation: suitable
protein purification: suitable (antibody labeling, concentration, enrichment)
L × diam.
12 cm (4.7 in.) × 2.97 cm
filtration area
1 cm2
working volume
10 mL
pore size
30 kDa NMWCO
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Features and Benefits
General description
Application
Other Notes
Physical form
Legal Information
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
flash_point_f
95.0 °F
flash_point_c
35 °C
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
bios-C-epdf-1043-1304-SP
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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