ACK5030NT
Amicon® Pro Affinity Concentration Kit Ni-NTA with 30kDa Amicon® Ultra-0.5 Device
Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.
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About This Item
material
Amicon Ultra styrene-butadiene device
Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding)
polyethylene cap
polyethylene frit
polypropylene device (for exchange)
polypropylene frit
polypropylene tube (AU0.5; for collection)
polypropylene tube (for collection)
polypropylene tube (holder)
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Pro
parameter
10 mL sample volume
technique(s)
buffer exchange: suitable
protein purification: suitable (antibody labeling)
protein purification: suitable (concentration)
protein purification: suitable (enrichment)
L
12 cm (4.7 in.)
diam.
2.97 cm
filtration area
1 cm2
working volume
10 mL
pore size
30 kDa NMWCO
shipped in
wet ice
General description
Application
Features and Benefits
Physical form
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C
Storage Class Code
3 - Flammable liquids
Flash Point(F)
95.9 °F
Flash Point(C)
35.5 °C
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
bios-C-epdf-1043-1304-SP
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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