Fluoro-Jade^® C

823

Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, were found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade is a registered trademark of Histo-Chem, Inc.

Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, were found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jadeâ dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence.



APPEARANCE: Coffee brown to brick red powder.

MOLECULAR WEIGHT: 823

EXCITATION PEAK: 485 nm

EMISSION PEAK: 525 nm

FILTER SYSTEM: Fluorescein / FITC

SOLUBILITY: Highly soluble in water and bases, moderately soluble in alcohol and weak acids.

TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.

FLUORO-JADE C has been used in:

• Fluoro-Jade C staining: to observe the pathological outcome of photothrombotic model after stroke
• to determine the direct neuronal death
• to evaluate neuronal degeneration in penumbra 
• for brain cells to identify the damaged cells
• to assess neuronal injury

The lyophilized powder should be stored well sealed at room temperature, preferable in a desiccator (due to its hygroscopic nature) for up to one year from date of receipt. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The .0002-.0001% working solution in 0.1% acetic acid should be used within 4 hrs of preparation.

Silica TLC (acetanitrile/water, 6/4) revealed the presence of two fluorescent spots, presumably corresponding to the di sulphate homologues. The presence of precursors or free fluorescein was not detected.

Replaces: AG325

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

FLUORO-JADE is a registered trademark of Histo-Chem, Inc.

Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.