Goat Anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed

Goat anti-Rabbit IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Rabbit IgG for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Suggested dilution for most applications:

1:500-1:5,000 for Immunoohistochemistry on tissue sections.

1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates.

1:10,000-1:200,000 for western blotting with chemiluminescent substrates.

Optimal working dilutions must be determined by the end user.

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.

RECONSTITUTION:

Reconstitute with 1.5 mL sterile distilled water.

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.