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Merck
CN

DR1045

Anti-GCLC Mouse mAb (3H1)

liquid, clone 3H1, Calbiochem®

Synonym(s):

Anti-Glutamate-Cysteine Ligase Catalytic Subunit

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About This Item

UNSPSC Code:
12352203
Clone:
3H1, monoclonal
Species reactivity:
human
Application:
—
Citations:
-

Key Documents

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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

3H1, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... GCLC(2729)

General description

Anti-GCLC, mouse monoclonal, clone 3H1, recognizes the ~70 kDa GCLC protein in HeLa and A431 cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
Purified mouse monolconal antibody. Recognizes the ~70 kDa GCLC protein.
Recognizes the ~70 kDa GCLC protein in HeLa and A431 cells.

Immunogen

A recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 &microg/ml)
Immunocytochemistry (10 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
A431 cells, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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