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Merck
CN

DR1052

Anti-SALL4 Mouse mAb (6E3)

liquid, clone 6E3, Calbiochem®

Synonym(s):

Anti-SAL-Like 4

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-SALL4 Mouse mAb (6E3), liquid, clone 6E3, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

6E3, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (0.2 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SALL4(57167)
mouse ... Sall4(99377)

Analysis Note

Positive Control
HeLa cells, NIH3T3 cells, Human testes tissue

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.2 g/ml)

Disclaimer

Toxicity: Regulatory Review (Z)

General description

Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
Recognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Immunogen

A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
NA

Other Notes

Cauffman, G., et al. 2009. Hum. Reprod.24 63.
Forte, A., et al. 2009. Mol. Med.15 392.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yanni Ma et al.
The EMBO journal, 34(3), 361-378 (2014-12-19)
MicroRNAs play important roles in controlling the embryonic stem cell (ESC) state. Although much is known about microRNAs maintaining ESC state, microRNAs that are responsible for promoting ESC differentiation are less reported. Here, by screening 40 microRNAs pre-selected by their

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