GF007
Insulin-like Growth Factor-II Protein, Recombinant human
The Insulin Like Growth Factor-II protein (or IGF-2 protein) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types.
Synonym(s):
IGF-II
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About This Item
eCl@ss:
32160405
UNSPSC Code:
12352207
biological source
human
assay
>98% (SDS-PAGE and HPLC)
specific activity
≥5 x 105 U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
input
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
human ... IGF2(3481)
General description
Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Human IGF-II is a 7.5 kDa protein containing 67 amino acid residues.
Product Source: E.coli
Application
For most in vitro applications, IGF-II exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Bone, muscle cells.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Physical form
Lyophilized powder with no additives
Preparation Note
The lyophilized IGF-II, though stable at room temperature, is best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted IGF-II should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.
Analysis Note
Specific Activity: Chemicon′s Human IGF-II is fully biologically active when compared to standards.The ED50 as determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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RECOMBINANT HUMAN INSULIN LIKE GROWTH FACTOR-II
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