Insulin-like Growth Factor-I Protein, Recombinant human

Insulin Like Growth Factor-I (IGF-I) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Human IGF-I is a 7.6 kDa protein containing 70 amino acid residues.

Insulin-like Growth Factor (IGF-1) is a polypeptide endocrine hormone similar in structure to insulin. Stimulated by growth hormone and produced primarily in the liver, it binds to the IGF-1 receptor with a high affinity and to the insulin receptor with a much lower affinity. The tyrosine kinase IGF-1 receptor is a potent activator of the Akt signaling pathway, which drives a variety of processes including cell growth and division and apoptosis inhibition. Because approximately 98% of IGF-1 is always bound to one of six binding proteins (IGF-BP), extraction is required to measure extracellular protein concentrations. Increased levels of IGF-1 have been correlated with an increased risk of cancer. In addition, while involved in reducing hemoglobin A1C levels and daily insulin consumption in types 1 and 2 diabetes, these increased levels have been also implicated in the development of diabetic retinopathy. Drugs have been developed as replacement therapy for severe primary IGF-1 deficiency, a group of diseases in which patients do not respond to growth hormone therapy.

Product Source: E.coli

For most in vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL. Responding cells are (partial list): bone, muscle cells, CHO cells.

Optimal working dilutions must be determined by end user.

Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 6, containing no additives.

The lyophilized IGF-I, though stable at room temperature, is best stored at -20°C for up to 12 months from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for one week or at -20°C for future use. Reconstituted IGF-I should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.

Specific Activity: Human IGF-I is fully biologically active when compared to standards.The ED50 as determined by a cell proliferation assay using FDC-P1 cells is ≤10.0 ng/mL

Replaces: GF006

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.