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GR01

Sigma-Aldrich

Anti-EGFR (Ab-1) Mouse mAb (528)

liquid, clone 528, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor

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100 μG
CN¥4,489.38

About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

CN¥4,489.38

Estimated to ship onSeptember 29, 2025Details

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

528, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Calbiochem®

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This Item
GR15LMABF119SAB4500718
Anti-EGFR (Ab-1) Mouse mAb (528) liquid, clone 528, Calbiochem®

Sigma-Aldrich

GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1) lyophilized, clone EGFR.1, Calbiochem®

Sigma-Aldrich

GR15L

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

Anti-EGFR Antibody, clone 528 (Azide-free) clone 528, from mouse

Sigma-Aldrich

MABF119

Anti-EGFR Antibody, clone 528 (Azide-free)

Anti-EGFR antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4500718

Anti-EGFR antibody produced in rabbit

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, rat, mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

528, monoclonal

clone

EGFR.1, monoclonal

clone

528, monoclonal

clone

polyclonal

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation(1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Negative Control
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue

Other Notes

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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