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Merck
CN

IL002

Sigma-Aldrich

Interleukin-2 Protein, Recombinant human

The recombinant human Interleukin-2 protein (or IL-2 protein) is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells.

Synonym(s):

IL-2

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160405
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biological source

human

Quality Level

Assay

>97% (SDS-PAGE and HPLC)
>98% (SDS-PAGE and HPLC)

mol wt

Mw 15.5 kDa

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg Endotoxin (of IL-2)
1 EU/μg

input

sample type hematopoietic stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... IL2(3558)

General description

Interleukin-2 (IL-2) is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Human IL-2 is a 15-16 kDa protein containing 159 amino acid residues. This preparation contains both monomeric and dimeric forms of IL-2.

Application

For most in vitro applications, IL-2 exerts its biological activity in the concentration range of 0.1 to 1.0 ng/mL. Responding cells are (partial list): T-cells, NK cells, LAK cells.

Preparation Note

The lyophilized IL-2, though stable at room temperature, is best stored at-20°C. After a quick spin reconstitute in 100mM acetic acid to a concentration of 0.1-1 mg/mL. This solution can be diluted into other buffered solutions or stored. Reconstituted IL-2 should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Runde Jiang et al.
Annals of translational medicine, 9(20), 1527-1527 (2021-11-19)
Our study aimed to investigate the effect of cancer-targeting gene-virotherapy and cytokine-induced killer (CIK) cell immunotherapy on lung cancer. CIK cells were obtained from peripheral blood mononuclear cells using interferon (IFN)-γ, interleukin (IL)-2, and CD3 monoclonal antibody. The CIK cells
Mehmet Sahin et al.
Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 47(3), 244-253 (2020-07-01)
Naturally occurring regulatory T cells (nTregs) are produced under thymic (tTregs) or peripherally induced (pTregs) conditions in vivo. On the other hand, Tregs generated from naive T cells in vitro under some circumstances, such as treatment with transforming growth factor-β

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