IPVH07850
Immobilon® -P PVDF Membrane
50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, transfer membrane
Synonym(s):
Western blotting membrane, blotting membrane, mini blots, transfer membrane
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About This Item
Product Name
Immobilon®-P PVDF Membrane, 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
material
PVDF membrane
plain filter
white filter
Quality Level
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable
western blot: suitable
filter L × W
7 cm × 8.4 cm
pore size
0.45 μm pore size
capacity
160 μg/cm2 adsorption capacity (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination
detection method
chemiluminescent
colorimetric
fluorometric
radioactive
shipped in
ambient
Related Categories
General description
Primary Binding Mechanisms: Electrostatic, hydrophobic;
Filter Code: IPVH
Application
Features and Benefits
- excellent protein retention
- high physical strength
- broad chemical compatibility
- suitable for a variety of staining applications and reprobing in immunodetection methods
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明
Related Content
Protein Blotting Survival Handbook: Tips and Tricks
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
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