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PureProteome Human Albumin/Immunoglobulin Depletion Kit

The PureProteome Human Albumin/Immunoglobulin Depletion Kit is a magnetic bead based kit that enables high depletion efficiency of Albumin and all Immunoglobulins from human serum or plasma samples.

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Synonym(s):
Protein Purification
eCl@ss:
32160405
NACRES:
NA.56

packaging

kit of 12 mL beads

manufacturer/tradename

PureProteome

technique(s)

depletion: suitable (human serum/plasma)
protein purification: suitable

particle size

10 μm

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

The PureProteome Human Albumin/Immunoglobulin Depletion Kit is a magnetic bead based kit that enables high depletion efficiency (typically >99%) of Albumin and all Immunoglobulins (i.e IgG, IgA, IgM, IgE and IgD) from human serum or plasma samples. The kit includes 12ml of premixed mag beads, 7ml of PBS buffer and an 8 pack of Amicon Ultra centrifugals for downstream buffer exchange or concentration if required.

Application

PureProteome Human Albumin/Immunoglobulin Depletion Kit has been used: to remove specific components from the serum sample for preparing protein stocks using capillary electrophoresis and depletion of all immunoglobulins from plasma.

Features and Benefits

  • Matrix comprises of magnetic silica beads coated with polymers and coupled with antibody ligands.
  • Beads have specific antibody ligands for recognizing and binding human serum albumin and immunoglobulins.
  • Assists in the detection and analysis of proteins of interest.

Components

12 mL of premixed magnetic beads, 7mL of PBS buffer, and an 8-pack of Amicon Ultra-2 mLcentrifugal filters, 3K MWCO

Analysis Note

Depletion: >98% albumin, IgG, IgA, IgM, IgE & IgD depletion. Typical values are >99% depletion.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Information

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Daniel J Sobczynski et al.
Bioengineering & translational medicine, 2(2), 180-190 (2017-09-22)
The high abundance of immunoglobulins (Igs) in the plasma protein corona on poly(lactic-co-glycolic) acid (PLGA)-based vascular-targeted carriers (VTCs) has previously been shown to reduce their adhesion to activated endothelial cells (aECs) in human blood flow. However, the relative role of
Semi-permanent cationic coating for protein separations
Crihfield CL, et al.
Journal of Chromatography A, 1607, (2019)
IgA and IgM protein primarily drive plasma corona-induced adhesion reduction of PLGA nanoparticles in human blood flow Daniel J. Sobczynski, Omolola Eniola-Adefeso
Sobczynski DJ and Eniola-Adefeso O
Bioengineering & translational medicine, 2(2), 180-190 (2017)

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