Coagulation factor VIII (UniProt: P00451; also known as Antihemophilic factor, AHF, Procoagulant component) is encoded by the F8 (also known as F8C) gene (Gene ID: 2157) in human. Factor VIII is a heavily glycosylated plasma protein that is synthesized with a signal peptide (aa 1-19) and is further cleaved and processed to generate Factor VIIIa heavy chain of 200 kDa (aa 20-1332), Factor VIIIa heavy chain of 92 kDa (aa 20-759), Factor VIII B chain (aa 760-1332), and the Factor VIIIa light chain (aa 1668-2351). In plasma, Factor VIII is thought to be associated in a complex with the highest molecular weight multimers of Von Willebrand protein in an inactive form and following injury or wound it dissociates from Willebrand factor and is activated (Factor VIIIa). Along with calcium and phospholipid, it acts as a cofactor for Factor IXa, which is responsible for converting Factor X to Xa. Its concentration in plasma ranges from 100-200 ng/ml.

Purified Human Factor VIII.

Anti-Factor VIII, 83 kDa light chain, clone F8C 27.4, Cat. No. MAB038-I, is a mouse monoclonal antibody that detects Factor VIII, 83 kDa light chain and is tested for use in ELISA and Western Blotting.

ELISA (indirect): (Dilution is assay based)

Western blotting (Dilution is assay based)

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone F8C 27.4 is a mouse monoclonal antibody that detects human Coagulation Factor VIII, 83 kDa light chain.

Purified mouse monoclonal in buffer containing PBS, pH 7.4, containing no preservatives.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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