Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

8.5 kDa

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer′s disease, Lewy bodies seen in Parkinson′s disease, and Pick bodies found in Pick′s disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde.

Research Category
Protein Trafficking

Neuroscience

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS) is validated for use in IH(P), IP, WB for the detection of Ubiquitin.

Immunohistochemistry (frozen or paraffin embedded sections):
1:250-1:1,000 dilution from a previous lot was used.

Electron Microscopy:
1:250-1:1,000 dilution from a previous lot was used.

Immunoprecipitation:
(Liang, 1995; Oberdorf, 1999)
Note: Suggested blocking agent is bovine serum albumin.

Immunoblotting:
1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).

Optimal working dilutions must be determined by end user.

Expected to cross-react with Bovine, Chicken, and Drosophila.

Reactivity with other species has not been confirmed.

Ubiquitin (76 amino acid, 8.5 kDa) both conjugated and unconjugated. Shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson’s disease) and Pick bodies (in Pick’s disease).

Mouse monoclonal IgG1κ in buffer containing containing 10 mM sodium azide.

Unpurified

Stable for 1 year at -20°C in undiluted aliquotes from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Alzheimer′s diseased brain.

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.

Replaces: 04-454

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