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Merck
CN

MAB16990

Anti-Hepatitis B Virus Antibody, core Antigen (ayw), clone 14E11, a.a. 134-140

ascites fluid, clone 14E11, Chemicon®

Synonym(s):

HBcAg

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Hepatitis B Virus Antibody, core Antigen (ayw), clone 14E11, a.a. 134-140, ascites fluid, clone 14E11, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

14E11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

shipped in

wet ice

Quality Level

300

Application

ELISA, immunoblotting, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells.

Recommended working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-Hepatitis B Virus Antibody, core antigen (ayw), clone 14E11, a.a. 134-140 is validated for use in ELISA, IF, IP, WB, IC, IH for the detection of Hepatitis B Virus.

Biochem/physiol Actions

Reacts with HBcAg (epitope recognized - amino acid positions 135-140).

SPECIES REACTIVITIES:

Cross-reacts with woodchuck hepatitis virus core antigen (WHcAg), but not with duck hepatitis B virus core antigen (DHBcAg).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Core Antigen (ayw)
Recombinant purified HBcAg from E.coli, SDS-denatured

Physical form

Ascites. Liquid, containing 0.1% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences
Sallberg, M, et al
The Journal of General Virology, 74 ( Pt 7), 1335-1340 (1993)
Boerries Brandenburg et al.
Hepatology (Baltimore, Md.), 42(6), 1300-1309 (2005-12-01)
Protein transduction domains (PTDs) have been used to deliver a variety of biologically active cargo across cellular membranes. However the potential of PTDs to mediate transport of nanoparticular structures into the cytoplasm bypassing the endosomal compartment remains unclear. Cell-permeable virus-like
The structure of the variable regions of mouse monoclonal antibodies to hepatitis B virus core antigen.
Skrivelis, V, et al.
Scandinavian Journal of Immunology, 37, 637-643 (1993)
Tobias Zahn et al.
Vaccine, 38(15), 3105-3120 (2020-03-03)
There are currently about 257 million people suffering from chronic HBV infection worldwide. In many cases, an insufficient Tcell response is causative for establishment of a chronic infection. To ensure a robust cellular immune response and induction of neutralizing antibodies
The affinities of monoclonal antibodies against core antigen of hepatitis B virus.
Apsalons, U and Bichko, V
Archives of Virology, 134, 393-402 (1994)
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