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MAB3104

Sigma-Aldrich

Anti-Calpain I Antibody, large subunit, clone P9

ascites fluid, clone P9, Chemicon®

Synonym(s):

mu-Calpain, μ-Calpain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

P9, monoclonal

species reactivity

hamster, mouse, human, rat

should not react with

canine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005186.2

UniProt accession no.

P07384

shipped in

dry ice

Gene Information

human ... CAPN1(823)

General description

Calpains are non-lysosomal cysteine proteeases which absolutely require calcium for activity. There are two major calpain isozymes: μ-calpain and m-calpain, which require micromolar or millimolar calicum for activity, respectively, in invitro assays. The major calpain isozymes are composed of two subunits: an 80kDa catalytic subunit; and a 30kDa regulatory subunit. The large and small subunits remain associated in the presence of calcium after proteolytic activation of the zymogen.

Specificity

Reacts with human calpain-I (μ-Calpain). P9 does not cross with human placental m-calpain (calpain II). This antibody will immunoprecipitate proteolytically active calpains.

Immunogen

Calpain-I large subunit from human erythrocyte calpain.
Epitope: large subunit

Application

Anti-Calpain I Antibody, large subunit, clone P9 is an antibody against Calpain I for use in ELISA, IP, WB & IC.
Immunoblot: 1:1,000 Detects an 80kDa large subunit μ-calpain, and will also detect the subunit in the non-activated proform of the zymogen, showing a band of ~110kDa. WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls. Immunocytochemistry: 1:100 on A431 epidermal carcinoma.

Immunoprecipitation

ELISA: 1:6,000 (against the immunogen)

Optimal working dilutions must be determined by the end user.
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

Physical form

Liquid

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
WI-38 human fibroblasts, or human erthryocytes are suggested as positive controls.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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