Skip to Content
Merck
CN
Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

i2H5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_000789.2
NM_152830.1
NM_152831.1

UniProt accession no.

P12821

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... AGT(183)

General description

The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.

Specificity

Angiotensin-converting enzyme [ACE] (N-terminal)

Immunogen

Angiotensin-converting enzyme from human lung

Application

Anti-Angiotensin Converting Enzyme Antibody, clone i2H5 is an antibody against Angiotensin Converting Enzyme for use in ELISA, IP, IC, IH.
Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

Immunocytochemistry: 1:100. Reacts with endothelial cells, macrophages (activated) and THP1 cells (monocyte line).

Immunoprecipitation: 1:1,000

ELISA (Stevens et al., 1990): 1:1,000

Structure-function of ACE (Danilov et al., 1994)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 100 mM borate buffer, pH 8.0 and 150 mM NaCl.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H360FD

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service